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Document Type |
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Article In Journal |
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Document Title |
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HISTOLOGICAL AND HISTOCHEMICAL EVALUA TION OF FURCAL تقييم هستولوجي وهستوكيميائي لاصلاح ثقوب تشعب الجذور باستخدام مادة (أم تي ايه) ممزوجة بمزلق أساسه الماء في الكلاب |
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Document Language |
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English |
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Abstract |
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Furcation perforation offers a great challenge to the dentist and causes poor prognosis of affected
teeth. Mineral trioxide aggregate (MTA) is a multipurpose material, that has many uses, such
as root end filling, pulp capping, and perforation repair. The present study was conducted to evaluate
histologically and histochemically the effect of a new MTA formulation when mixed with Surgilube
gel@on repair of furcation perforation. Eight dogs were included in this study. Access cavities
were performed in mandibular premolars. One side of the mandible was used as experimental
group, where the furcation perforations in four premolars were immediately sealed with a mixture
of MTA and Surgilube gel. In the other side of the mandible, two premolars were used as positive
controls where perforations were left unsealed, whereas the other two premolars were negative controls
left intact without perforation. Clinical examination was performed at baseline and before euthanizing
the animals. Four dogs were sacrificed after one month, whereas the remaining were killed
after four months. Histologic evaluation was done using H & E and Trichrome stains, as well as histochemical
examination for detection of alkaline phosphatase (ALP) enzyme activity. Negative controls
showed normal healing clinically with neither pocket formation nor radiographic bone loss.
This was confirmed histologically and histochemically. As for the MTA group, the same clinical
'findings were observed. Histologic evaluation at one month revealed mild inflammation, and appearance
of early signs of regeneration including formation of new cementum and alveolar bone.
Tissue maturation and calcification were evident at four months in this group with disappearance of
inflammatory reaction. The perforations were sealed with periodontal attachment apparatus reformed
at that site. Concerning unsealed perforations, they were associated with pocket formation.
Histologically, they showed at one month severe inflammatory reaction with formation of fibrous
tissue, whereas at four months, inflammation was still persisting with incomplete healing evidenced
by fibrous tissue, irregular woven bone and small amount of new cementum. Histochemical evaluation
revealed more intense reaction of ALP activity in MTA group, that was decreased at four
months compared to one month as an indication of tissue mineralization. According to this study,
MTA mixed with Surgilube gel is an excellent repair material for furcation perforations that has the
potential of periodontal regeneration. |
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ISSN |
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0 |
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Journal Name |
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Egyptian Dental Journal |
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Volume |
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52 |
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Issue Number |
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2 |
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Publishing Year |
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1426 AH
2006 AD |
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Article Type |
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Article |
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Added Date |
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Monday, October 18, 2010 |
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Researchers
| غادة منصور | Mansour, Ghada | Investigator | Doctorate | |
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