Document Details
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Document Type |
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Article In Journal |
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Document Title |
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IN VITRO, CLINICAL AND IMMUNOCHEMICAL EVALUATION OF A LOCAL DRUG DELIVERY SYSTEM DOXYCYCLINE HYCLATE IN THE MANAGEMENT OF SEVERE CHRONIC PERIODONTITIS تقييم معملي واكلينيكي وكميائي مناعي لنظام التوصيل الموضعي لعقار الدوكسى سيكلين هيكلات لعلاج مرضى التهاب النسيج حول السنى المزمن ذى الدرجة المتوسطة الى الشديدة |
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Document Language |
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English |
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Abstract |
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The purpose of this study was to evaluate
in vitro, clinically and immunochemically the
effectiveness of two doxycycline gel
fommlations based on chitosan (Ch) and
pluronic (PI) polymers in management of
moderate to severe chronic periodontitis cases.
25% Doxy Pluronic gel and 2% Doxy
chitosan gel were prepared in faculty of
Pharmacy, Alexandria University. They were
submitted to an in vitro study ,including
evaluation of their viscosity , injectability,
Doxy drug release and gel dissolution. PI
changed into stiff gel at temperature around
~210 C. Change of pH from 4.4 to
physiological pH, and changes in Ch solubility
did not influence its viscosity The
injectability results showed that PI was
expelled from the syringe under the influence
of a weight < 2kg; whereas the Ch gel needed
about 3.7 kg. Release from PI 25% gel formula
indicated drug release of ~ 18% after 1 hour,
and ~26% after 7 hours. Cumulative Doxy
release profile from Ch was about 30% after 1
hour and 48% after 7 hours. Ch dissolution
profile showed initial lag period followed by
slow dissolution in the first 4 hours. Ch 2%
revealed significantly higher %dissolution
efficiency than PI 25%, (36.77% and 20.93%
respectively).The clinical and immunochemical studies were conducted on 15 patients with
moderate to severe chronic periodontitis. 45
matched pockets in these patients (3 in each
patient) located anteriorly on single rooted
teeth constituted the study sample. All patients
received scaling and root planning. Qualifying
defects were randomly assigned as either
receiving subgingival chitosan based Doxy
gel, pluronic based Doxy gel or placebo gel.
At baseline, 6 weeks and 3 months posttherapy,
clinical evaluation included plaque
scores (PI), papillary bleeding scores (PBI),
probing depth (PD), and clinical attachment
level (CAL).Post treatment results showed a
significant improvement in all studied
variables at 6 weeks and 3 months when
compared to baseline in terms of plaque index
reduction (PIR), papillary bleeding reduction
(PBIR), pocket depth reduction (PDR),and
gain in attachment levels (ALG), except for
placebo group that did not show significant
ALG post therapy. Intergroup data analysis
showed significantly better results in Doxy
groups when compared to placebo group, with
chitosan treated sites manifesting the best
clinical improvement.For the immunochemical
study, GCF samples were withdrawn from
treated sites and were evaluated using ELISA
for crevicular elastase level. For all patients,
GCF elastase decreased significantly following
therapy, with Doxy groups revealing statistically significant reduction In
comparison to placebo group, and chitosan
treated sites having significantly decreased
elastase level than pluronic treated sites. In
conclusion, both chitosan and pluronic showed
promising results as polymers used for
controlled release intra-pocket doxycycline
therapy, both in vitro, clinically and
immunochemically. |
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ISSN |
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0 |
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Journal Name |
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ِِAlexandria Dental Journal |
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Volume |
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29 |
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Issue Number |
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4 |
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Publishing Year |
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1424 AH
2004 AD |
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Article Type |
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Article |
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Added Date |
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Tuesday, October 19, 2010 |
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Researchers
| غادة منصور | Mansour, Ghada | Investigator | Doctorate | |
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